[ Link to paper (PDF) ] [Commentary in Science ] [ Highlights in Nat Rev Mol Cell Biol. ] [ Faculty of 1000 ] [ Back to home page ]Han X, Wang CT, Bai J, Chapman ER, Jackson MB. Transmembrane segments of syntaxin line the fusion pore of Ca2+-triggered exocytosis. Science, 2004 Apr 9; 304(5668):289-92.
Transmembrane segments of syntaxin line the fusion pore of Ca2+-triggered exocytosis.
The fusion pore of regulated exocytosis is a channel that connects and spans
the vesicle and plasma membranes. The molecular composition of this important
intermediate structure of exocytosis is unknown. Here, we found that mutations
of some residues within the transmembrane segment of syntaxin (Syx), a plasma
membrane protein essential for exocytosis, altered neurotransmitter flux through
fusion pores and altered pore conductance. The residues that influenced
fusion-pore flux lay along one face of an alpha-helical model. Thus, the fusion
pore is formed at least in part by a circular arrangement of 5 to 8 Syx
transmembrane segments in the plasma membrane.